A DIRECT METHOD FOR COUNTING BACTERIA IN FECES

A method for counting acid-fast bacteria.

A reliable, convenient, and accurate method for counting ac id-fast hacteria ( AFB) is essential for much work with Mycobacterium leprae. The procedure used , previously in the laboratory (~, 4 ) had two technical difficulties: (a) In order for the drops to spread satisfactorily the microscope slides had to have a uniform wetability of the correct degree; sometimes this requirement was difficul...

The Direct or Breed Method for Counting Bacteria in Tomato Catsup, Pulp or Paste.

The method used for counting bacteria in tomato catsups, pulp or paste depends on the use of the Zeiss blood counter. In this method a drop of catsup diluted with two portions of distilled water is placed in the counting chamber so as to cover the space within the moat. This stands for fifteen minutes before counting.and the bacteria in 25 small squares are then counted and an average is obtain...

A Quantitative Differential Method for Counting Mixed Cultures of Bacteria.

New method for counting bacteria associated with coral mucus.

The ability to count bacteria associated with reef-building corals in a rapid, reliable, and cost-effective manner has been hindered by the viscous and highly autofluorescent nature of the coral mucus layer (CML) in which they live. We present a new method that disperses bacterial cells by trypsinization prior to 4',6-diamidino-2-phenylindole (DAPI) staining and quantification by epifluorescenc...

Improved method for direct counting of basophil leucocytes.

An improved method for the direct counting of basophil leucocytes from venous or capillary blood is described. The method is based upon the use of cetylpyridinium chloride to lyse the erythrocytes and render the muco-polysaccharides of the granule insoluble. Aluminium sulphate is used to mordant toluidine blue to the granules. Our knowledge of the role of the basophil leucocytes in disease has ...